Ergebnisse 25 – 36 von 76 werden angezeigt

  • T4 DNA Ligase for DNA klonierung Polydeoxyribonucleotide synthase

    T4 DNA Ligase

    T4 DNA Ligase (2.5 Weiss*units/μL and 6.0 Weiss/units/uL)

    • T4 DNA Ligase catalyses the formation of a phosphodiester bond between neighbouring 5′ -phosphate and 3′ -hydroxyl termini in duplex DNA or RNA
    • Covalently joins DNA fragments with complementary cohesive ends or blunt ends
    • Cloning of PCR and restriction fragments
    • Self circularisation of linear DNA
    • Ligation of adaptors or linkers to DNA
    • Repair of single stranded breaks (nicks)

     

    (*Note: one Weiss unit is equivalent to about 67 cohesive end ligation units)

    not rated 36,30 64,20 

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  • Orange DNA Loading Dye

    6x Orange DNA Loading Buffer

    Store at room temperature for up to 12 months. For longer periods, store at -20°C.

     

    6x Orange DNA Loading Buffer

    Eco-friendly DNA loading dye

    • Free from persistent organohalogen compounds like and bromophenol blue and and the common desity reagent sucrose-epichlorhydrin-copolymers.
    • The Orange G tracking dye, comigrates with approximately 50bp dsDNA on 1% TAE or TBE Gel
    • No DNA masking on UV or blue light
    • SDS efficiently stops restriction digests and nucleases
    • Reduced EDTA content for clear and sharp bands
    • Optimised glycerol content for easy loading

    not rated 9,20 18,50 

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  • Lambda DNA EcoRI/HindIII Marker

    Lambda DNA EcoRI/HindIII Marker Lambda Phage DNA completely digested with EcoR I and Hind III. The band pattern shows 13 discrete bands: 21226*, 5148, 4973, 4268, 3530*, 2027, 1904, 1584,1375, 947, 831, 564, 125. *The cohesive ends (12b cos site … Read More
    not rated 22,90 33,80 

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  • Lambda Bakteriophagen DNA
    not rated 19,90 27,40 

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  • 100 bp DNA Size Marker

    100bp DNA ladder

    • 8 discrete fragments (in base pairs) 100, 200, 300, 400, 500, 600, 800, 1000
    • 500bp and 1000bp bands with increased intensity
    not rated 33,20 132,80 

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  • NheI Restriction enzyme

    NheI

    NheI is a restriction enzyme purified from Neisseria mucosa heidelbergensis that cleaves the palindromic sequence G▼CTAGC (ATCC 25999).

    Concentration: 10U/µL
    Source: Neisseria mucosa var. heidelbergensis Berger
    Reagents supplied with: 10x Simple Buffer.
    The Enzyme is supplied with 10x universal Simple Buffer. The Enzyme is also active in universal potassium glutamate or potassium acetate buffers from other suppliers like theCutSmart® Buffer from NEB and Tango™ Buffer from Thermo Fisher Scientific (Fermentas) and can combined with enzymes from other suppliers in double digests.
    Store at: -20 °C

    not rated 50,00 189,60 

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  • SacI Isochizomer

    SstI (SacI)

    SstI is a restriction enzyme purified from Streptomyces stanford that cleaves the palindromic sequence GAGCT▼C.

    Isoschizomer: SacI

    Concentration: 10U/µL
    Source: Streptomyces stanford
    Reagents supplied with: 10x Buffer L and the universal 10x Simple Buffer for double digests.
    Store at: -20 °C

     

    not rated 31,40 229,70 

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  • SseBI StuI Isoschizomer

    SseBI (StuI)

    SseBI is a restriction enzyme purified from Streptomyces species that cleaves the palindromic sequence AGG▼CCT.

    Prototype (Isoschizomer): StuI

    Concentration: 10U/µL

    Source: Streptomyces species

    Reagents supplied with: 10x Buffer H and the universal 10x Simple Buffer for double digests.

    Store at: -20 °C

    not rated 29,40 199,90 

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  • SlaI Restriction enzyme

    SlaI (XhoI)

    SlaI is a restriction enzyme purified from Streptomyces lavendulae that cleaves the palindromic sequence CTCGAG.

    Prototype (Isoschizomer): XhoI

    Concentration: 10U/µL
    Source: Stremptomyces lavendulae
    Reagents supplied with: Buffer SH and the universal Simple Buffer for double digests. SlaI is also active in CutSmart® Buffer from NEB, 2x Tango™ Buffer from Thermo Fisher Scientific (Fermentas) or high salt buffers from other suppliers as long as the buffer is supplemented with 100µg/mL BSA.
    Store at: -20 °C

    not rated 29,20 78,70 

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  • PstI restriction endonucleases

    PstI

    PstI is a restriction enzyme purified from a recombinant E. coli strain that carries a plasmid encoding the PstI gene from Providencia stuartii 164. PstI cleaves the palindromic DNA sequence CTGCAG.

    Concentration: 10U/µL
    Source: A E. coli strain that carries the cloned PstI gene from Providencia stuartii.
    Reagents supplied with: Buffer UniquePstI and the universal Simple Buffer for double digests.
    Store at: -20 °C

     

    not rated 30,80 124,90 

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  • NotI Restriktionsendonuklease

    NotI

    NotI is a restriction enzyme purified from Nocardia otitidis-caviarum that cleaves the palindromic sequence GCGGCCGC.

    Concentration: 10U/µL

    Source: Nocardia corallina

    Reagents supplied with: The Enzyme is supplied with 10x UniqueNotI Buffer. The enzyme is also fully active in the respective buffers from other suppliers.

    Store at: -20 °C

    Qualified for rapid digest in just 15 minutes.

    not rated 49,90 388,50 

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  • HindIII

    HindIII is a restriction enzyme is a restriction enzyme purified from a recombinant E. coli that carries a plasmid encoding the HindIII gene from Haemophilus influenzae. HindIII cleaves the palindromic sequence AAGCTT.

    Concentration: 10U/µL
    Source: Haemophilus influenzae Rd
    Reagents supplied with:  10x buffer M and the 10x universal Simple Buffer for double digests. The enzyme is also fully active in the respective buffers from other suppliers and may be used in double digests together with enzymes from other suppliers.
    Store at: -20 °C

    not rated 29,90 129,00 

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