blunting

Concentration: 5U/µL

• Highly pure recombinat enzyme (>98 % as indicated by SDS-PAGE)
• Lacks the 5´->3´ exonuclease activity
• Retains polymerase and 3’→5’exonuclease activity (proofreading, blunting)
• Fill-in of 5’ overhangs to form blunt ends (blunting)
• Second strand cDNA synthesis
• Probe labelling, using random primers

Source: E. coli strain carrying a plasmid with a clone copy of DNA Polymerase I large fragment
Reagents supplied with: 10x Klenow reaction buffer
Store at: -20 °C