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  • Taq DNA Polymerase, recombinant

    • Best choice for colony PCR
    • Adds a single deoxyadenosine (A) to the 3′- ends of PCR products
    • No detectable 3’→5′ proofreading exonuclease activity
    • For standard PCR where proofreading is not required
    • Excellent cost-performance ratio 

    Concentration: 5U/µL
    Source: Purified from an E. coli strain carrying a plasmid with Taq DNA polymerase gene from Thermus aquaticus YT-1.
    Reagents supplied with: 10x Taq polymerase buffer
    Store at: -20 °C

    not rated 31,00 379,00 

    exkl. MwSt.

    zzgl. Versandkosten

    Lieferzeit: Lieferzeit 1-2 Werktage

    Details
  • Klenow Enzyme (DNA Polymerase I Large Fragment)

    Concentration: 5U/µL

    • Highly pure recombinat enzyme (>98 % as indicated by SDS-PAGE)
    • Lacks the 5´->3´ exonuclease activity
    • Retains polymerase and 3’→5’exonuclease activity (proofreading, blunting)
    • Fill-in of 5’ overhangs to form blunt ends (blunting)
    • Second strand cDNA synthesis
    • Probe labelling, using random primers

    Source: E. coli strain carrying a plasmid with a clone copy of DNA Polymerase I large fragment
    Reagents supplied with: 10x Klenow reaction buffer
    Store at: -20 °C

    not rated 42,10 169,50 

    exkl. MwSt.

    zzgl. Versandkosten

    Lieferzeit: Lieferzeit 1-2 Werktage

    Details