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  • T4-DNA-Ligase


     (2.5 Weiss units/μL and 6.0 Weiss/units/uL)

    (Note: one Weiss unit is equivalent to about 67 cohesive end ligation units)

    • T4 DNA Ligase catalyses the formation of a phosphodiester bond between neighbouring 5′ -phosphate and 3′ -hydroxyl termini in duplex DNA or RNA
    • Covalently joins DNA fragments with complementary cohesive ends or blunt ends
    • Repairs single stranded nicks
    not rated 36,30 64,20 

    exkl. MwSt.

    zzgl. Versandkosten

    Lieferzeit: 2-3 Tage

  • Klenow Enzyme (DNA Polymerase I Large Fragment)

    Concentration: 5U/µL

    • Highly pure recombinat enzyme (>98 % as indicated by SDS-PAGE)
    • Lacks the 5´->3´ exonuclease activity
    • Retains polymerase and 3’→5’exonuclease activity (proofreading, blunting)
    • Fill-in of 5’ overhangs to form blunt ends (blunting)
    • Second strand cDNA synthesis
    • Probe labelling, using random primers

    Source: E. coli strain carrying a plasmid with a clone copy of DNA Polymerase I large fragment
    Reagents supplied with: 10x Klenow reaction buffer
    Store at: -20 °C

    not rated 42,10 169,50 

    exkl. MwSt.

    zzgl. Versandkosten

    Lieferzeit: Lieferzeit 1-2 Werktage