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  • T4 DNA Ligase for DNA klonierung Polydeoxyribonucleotide synthase

    T4 DNA Ligase

    T4 DNA Ligase (2.5 Weiss*units/μL and 6.0 Weiss/units/uL)

    • T4 DNA Ligase catalyses the formation of a phosphodiester bond between neighbouring 5′ -phosphate and 3′ -hydroxyl termini in duplex DNA or RNA
    • Covalently joins DNA fragments with complementary cohesive ends or blunt ends
    • Cloning of PCR and restriction fragments
    • Self circularisation of linear DNA
    • Ligation of adaptors or linkers to DNA
    • Repair of single stranded breaks (nicks)

     

    (*Note: one Weiss unit is equivalent to about 67 cohesive end ligation units)

    not rated 36,30 64,20 

    exkl. MwSt.

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    Lieferzeit: Lieferzeit 1-2 Werktage

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  • Quick Ligation Kit Simplebiotech Schnellligations Kit

    Rapid DNA Ligation Kit

    Rapid DNA Ligation Kit for fast cloning at affordable price

    • Quick: DNA ligation of blunt or sticky DNA ends in just 15min or less
    • Convienient ligation at room temperature
    • Improved ligation of blunt end DNA
    • Direct transformation of chemical competent e. coli cells 

    (For transformation of electrocometent e. coli cells the ligation product should be purifed before transformation)

    not rated 49,90 78,40 

    exkl. MwSt.

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    Lieferzeit: Lieferzeit 1-2 Werktage

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  • Thermocycler, GTC96S, PCR Maschine, Thermal Cycler, PCR Gerät, Thermal Cycler GTC96S, Termociclador, PCR Block, Cleaver Scientiffic PCR Gerät,

    Thermocycler 96 Well PCR- GTC96S Cleaver Thermal Cycler

    The Cleaver Scientific GTC96S Thermal Cycler is compatible with all standard 96 plates and 0.2 ml PCR tubes. The wells for the tubes can be divided into six discrete (4×4 well) temperature zones, easily distinguished by blue and black squares. … Read More
    not rated 4.639,00 

    exkl. 19 % MwSt.

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    Lieferzeit: ca. 4 Wochen

    Details
  • Klenow Enzyme (DNA Polymerase I Large Fragment)

    Concentration: 5U/µL

    • Highly pure recombinat enzyme (>98 % as indicated by SDS-PAGE)
    • Lacks the 5´->3´ exonuclease activity
    • Retains polymerase and 3’→5’exonuclease activity (proofreading, blunting)
    • Fill-in of 5’ overhangs to form blunt ends (blunting)
    • Second strand cDNA synthesis
    • Probe labelling, using random primers

    Source: E. coli strain carrying a plasmid with a clone copy of DNA Polymerase I large fragment
    Reagents supplied with: 10x Klenow reaction buffer
    Store at: -20 °C

    not rated 42,10 169,50 

    exkl. MwSt.

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    Lieferzeit: Lieferzeit 1-2 Werktage

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