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not rated 36,30 € – 64,20 €T4 DNA Ligase
T4 DNA Ligase (2.5 Weiss*units/μL and 6.0 Weiss/units/uL)
- T4 DNA Ligase catalyses the formation of a phosphodiester bond between neighbouring 5′ -phosphate and 3′ -hydroxyl termini in duplex DNA or RNA
- Covalently joins DNA fragments with complementary cohesive ends or blunt ends
- Cloning of PCR and restriction fragments
- Self circularisation of linear DNA
- Ligation of adaptors or linkers to DNA
- Repair of single stranded breaks (nicks)
(*Note: one Weiss unit is equivalent to about 67 cohesive end ligation units)
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not rated 4.639,00 €Thermocycler 96 Well PCR- GTC96S Cleaver Thermal Cycler
The Cleaver Scientific GTC96S Thermal Cycler is compatible with all standard 96 plates and 0.2 ml PCR tubes. The wells for the tubes can be divided into six discrete (4×4 well) temperature zones, easily distinguished by blue and black squares. … Read More -
not rated 49,90 € – 78,40 €Rapid DNA Ligation Kit
Rapid DNA Ligation Kit for fast cloning at affordable price
- Quick: DNA ligation of blunt or sticky DNA ends in just 15min or less
- Convienient ligation at room temperature
- Improved ligation of blunt end DNA
- Direct transformation of chemical competent e. coli cells
(For transformation of electrocometent e. coli cells the ligation product should be purifed before transformation)
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not rated 42,10 € – 169,50 €Klenow Enzyme (DNA Polymerase I Large Fragment)
Concentration: 5U/µL
• Highly pure recombinat enzyme (>98 % as indicated by SDS-PAGE)
• Lacks the 5´->3´ exonuclease activity
• Retains polymerase and 3’→5’exonuclease activity (proofreading, blunting)
• Fill-in of 5’ overhangs to form blunt ends (blunting)
• Second strand cDNA synthesis
• Probe labelling, using random primersSource: E. coli strain carrying a plasmid with a clone copy of DNA Polymerase I large fragment
Reagents supplied with: 10x Klenow reaction buffer
Store at: -20 °C
