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    Taq DNA Polymerase, recombinant

    Taq DNA Polymerase is now supplied with -inhibitor free- DNA Loading buffer IF

    • Best choice for colony PCR
    • Adds a single deoxyadenosine (A) to the 3′- ends of PCR products
    • No detectable 3’→5′ proofreading exonuclease activity
    • For standard PCR where proofreading is not required
    • Excellent cost-performance ratio 

    Concentration: 5U/µL
    Source: Purified from an E. coli strain carrying a plasmid with Taq DNA polymerase gene from Thermus aquaticus YT-1.
    Reagents supplied with: 10x Taq polymerase buffer and 10x DNA Loading buffer IF -inhibitor free-
    Store at: -20 °C

    not rated 31,00 379,00 

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  • Bovine Serum Albumin

    BSA, non acetylated Bovine Serum Albumin, Molecular Biology Grade

    Bovine Serum Albumin (BSA), non-acetylated

    molecular biology grade 20mg/mL (Tris buffered 50% Glycerol)

    Highly pure non-acetylated not chemically modified BSA for use as enzyme stabiliser

    • Stabilizes enzymes during incubation and storage
    • Reduces adhesion of proteins to tubes and pipet tips
    • Improves PCR amplification of low purity templates (unlike acetylated BSA, that inhibits amplification)
    • Improves DNA restriction digests and ligation with T4 DNA ligase

    Note: This product is not recommended as mass standard for protein determination or size standard for protein electrophoresis.

    not rated 29,20 99,40 

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  • BglII

    Bgl II is a restriction enzyme that cleaves the palindromic sequence A▼GATCT. The Enzyme is purified from a Bacillus globigii strain lacking Bgl I.

    Concentration: 10U/µL

    Reagents supplied with: The Enzyme is supplied with 10x Buffer H and the universal 10x Simple Buffer for double digests. The Enzyme is also active in the respective buffers from other suppliers can be combined with enzymes from other suppliers in double digests.
    Store at: -20 °C

    not rated 34,70 143,20 

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  • EcoRI

    EcoRI is a restriction enzyme isolated from E. coli RY 13 that cleaves the palindromic sequence G▼AATTC.

    Concentration: 10U/µL
    Source: Escherichia coli RY13
    Reagents supplied with: The Enzyme is supplied with 10x UniqueEcoRI Buffer (recommended) and the universal Simple Buffer for double digests. The enzyme is also fully active in the respective buffers from other suppliers.

    Store at: -20 °C

    Star activity: Conditions of low ionic strength, high enzyme concentration, glycerol concentration or pH>8.0 may result in star activity.

     

    not rated 36,60 143,50 

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  • Orange DNA Loading Dye

    6x Orange DNA Loading Buffer

    Store at room temperature for up to 12 months. For longer periods, store at -20°C.

     

    6x Orange DNA Loading Buffer

    Eco-friendly DNA loading dye

    • Free from persistent organohalogen compounds like and bromophenol blue and and the common desity reagent sucrose-epichlorhydrin-copolymers.
    • The Orange G tracking dye, comigrates with approximately 50bp dsDNA on 1% TAE or TBE Gel
    • No DNA masking on UV or blue light
    • SDS efficiently stops restriction digests and nucleases
    • Reduced EDTA content for clear and sharp bands
    • Optimised glycerol content for easy loading

    not rated 9,20 18,50 

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  • NheI

    NheI is a restriction enzyme purified from Neisseria mucosa heidelbergensis that cleaves the palindromic sequence G▼CTAGC (ATCC 25999).

    Concentration: 10U/µL
    Source: Neisseria mucosa var. heidelbergensis Berger
    Reagents supplied with: 10x Simple Buffer.
    The Enzyme is supplied with 10x universal Simple Buffer. The Enzyme is also active in universal potassium glutamate or potassium acetate buffers from other suppliers like theCutSmart® Buffer from NEB and Tango™ Buffer from Thermo Fisher Scientific (Fermentas) and can combined with enzymes from other suppliers in double digests.
    Store at: -20 °C

    not rated 50,00 189,60 

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  • SacI Isochizomer

    SstI (SacI)

    SstI is a restriction enzyme purified from Streptomyces stanford that cleaves the palindromic sequence GAGCT▼C.

    Isoschizomer: SacI

    Concentration: 10U/µL
    Source: Streptomyces stanford
    Reagents supplied with: 10x Buffer L and the universal 10x Simple Buffer for double digests.
    Store at: -20 °C

     

    not rated 31,40 229,70 

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  • SseBI (StuI)

    SseBI is a restriction enzyme purified from Streptomyces species that cleaves the palindromic sequence AGG▼CCT.

    Prototype (Isoschizomer): StuI

    Concentration: 10U/µL

    Source: Streptomyces species

    Reagents supplied with: 10x Buffer H and the universal 10x Simple Buffer for double digests.

    Store at: -20 °C

    not rated 29,40 199,90 

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  • SlaI (XhoI)

    SlaI is a restriction enzyme purified from Streptomyces lavendulae that cleaves the palindromic sequence CTCGAG.

    Prototype (Isoschizomer): XhoI

    Concentration: 10U/µL
    Source: Stremptomyces lavendulae
    Reagents supplied with: Buffer SH and the universal Simple Buffer for double digests. SlaI is also active in CutSmart® Buffer from NEB, 2x Tango™ Buffer from Thermo Fisher Scientific (Fermentas) or high salt buffers from other suppliers as long as the buffer is supplemented with 100µg/mL BSA.
    Store at: -20 °C

    not rated 29,20 78,70 

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  • PstI

    PstI is a restriction enzyme purified from a recombinant E. coli strain that carries a plasmid encoding the PstI gene from Providencia stuartii 164. PstI cleaves the palindromic DNA sequence CTGCAG.

    Concentration: 10U/µL
    Source: A E. coli strain that carries the cloned PstI gene from Providencia stuartii.
    Reagents supplied with: Buffer UniquePstI and the universal Simple Buffer for double digests.
    Store at: -20 °C

     

    not rated 30,80 124,90 

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  • NotI

    NotI is a restriction enzyme purified from Nocardia otitidis-caviarum that cleaves the palindromic sequence GCGGCCGC.

    Concentration: 10U/µL

    Source: Nocardia corallina

    Reagents supplied with: The Enzyme is supplied with 10x UniqueNotI Buffer. The enzyme is also fully active in the respective buffers from other suppliers.

    Store at: -20 °C

    Qualified for rapid digest in just 15 minutes.

    not rated 49,90 388,50 

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  • Simplebiotech Schnellligations Kit

    Rapid DNA Ligation Kit

    Rapid DNA Ligation Kit for fast cloning at affordable price

    • Quick: DNA ligation of blunt or sticky DNA ends in just 15min or less
    • Convienient ligation at room temperature
    • Improved ligation of blunt end DNA
    • Direct transformation of chemical competent e. coli cells 

    (For transformation of electrocometent e. coli cells the ligation product should be purifed before transformation)

    not rated 49,90 78,40 

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  • Enzyme friendly DNA loading Dye

    10x DNA Loading Buffer IF -inhibitor free-

    10x DNA Loading Buffer IF

    -Inhibitor free-

    Store at: -20 °C

    This DNA Loading Buffer contains orange G for easy tracking of DNA in agarose without masking your DNA bands and a propritary density reagent. It is free from enzyme inhibitors like SDS and EDTA and persistent organochlorine compounds like sucrose-epichlorhydrin copolymers. The buffer does not inhibit PCR reactions.

    not rated 16,40 37,00 

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  • T4-Ligase

    T4 DNA Ligase

    T4 DNA Ligase (2.5 Weiss*units/μL and 6.0 Weiss/units/uL)

    • T4 DNA Ligase catalyses the formation of a phosphodiester bond between neighbouring 5′ -phosphate and 3′ -hydroxyl termini in duplex DNA or RNA
    • Covalently joins DNA fragments with complementary cohesive ends or blunt ends
    • Cloning of PCR and restriction fragments
    • Self circularisation of linear DNA
    • Ligation of adaptors or linkers to DNA
    • Repair of single stranded breaks (nicks)

     

    (*Note: one Weiss unit is equivalent to about 67 cohesive end ligation units)

    not rated 36,30 64,20 

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  • ATP 10mM pH 7,5 mit Tris base

    ATP (Adenosine 5´-Triphosphate), 10mM

    ATP (Adenosine 5′-Triphosphate, 10mM)

                >98 % Purity

                Adjusted to pH 7.5 with Tris base (can be stored at -20 °C for 12 month)

    not rated 17,80 24,70 

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  • Adenosin 5' Triphosphate

    ATP (Adenosine 5´-Triphosphate), 100mM

    ATP (Adenosine 5′-Triphosphate, 100mM

                >98 % Purity

                Adjusted to pH 7.5 with Tris base (can be stored at -20 °C for 12 month)

    not rated 49,20 

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  • Lambda DNA EcoRI/HindIII Marker

    Lambda DNA EcoRI/HindIII Marker Lambda Phage DNA completely digested with EcoR I and Hind III. The band pattern shows 13 discrete bands: 21226*, 5148, 4973, 4268, 3530*, 2027, 1904, 1584,1375, 947, 831, 564, 125. *The cohesive ends (12b cos site … Read More
    not rated 22,90 33,80 

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  • not rated 19,90 27,40 

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  • 1 kb DNA Size Marker

    1kb DNA Ladder

    (0.5μg/μl)

    • 7 discrete fragments (in kilobases) 1, 2, 3, 4, 5, 6, 8
    • 4kb band with increased intensity
    not rated 32,50 130,00 

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  • 100 bp DNA Size Marker

    100bp DNA ladder

    • 8 discrete fragments (in base pairs) 100, 200, 300, 400, 500, 600, 800, 1000
    • 500bp and 1000bp bands with increased intensity
    not rated 33,20 132,80 

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  • Klenow Enzyme (DNA Polymerase I Large Fragment)

    Concentration: 5U/µL

    • Highly pure recombinat enzyme (>98 % as indicated by SDS-PAGE)
    • Lacks the 5´->3´ exonuclease activity
    • Retains polymerase and 3’→5’exonuclease activity (proofreading, blunting)
    • Fill-in of 5’ overhangs to form blunt ends (blunting)
    • Second strand cDNA synthesis
    • Probe labelling, using random primers

    Source: E. coli strain carrying a plasmid with a clone copy of DNA Polymerase I large fragment
    Reagents supplied with: 10x Klenow reaction buffer
    Store at: -20 °C

    not rated 42,10 169,50 

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  • ScaI

    ScaI is a restriction enzyme purified from Streptomyces caespitosus that cleaves the palindromic sequence AGT▼ACT creating blunt ends.

    Concentration: 10U/µL
    Source: Streptomyces caespitosus
    Reagents supplied with: Buffer 10x UScaI
    The Enzyme is also active in respective buffers from other suppliers and can combined with enzymes from other suppliers in double digests.
    Store at: -20 °C

    not rated 33,50 132,80 

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  • SalI

    SalI is a restriction enzyme purified from Streptomyces albus G. that cleaves the palindromic sequence G▼TCGAC.

    Concentration: 10U/µL
    Source: Streptomyces albus G

    Reagents supplied with: Buffer SH and the universal Simple Buffer for double digests.
    Store at: -20 °C

     

    not rated 39,80 69,90 

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  • PvuII

    PvuII is a restriction enzyme purified from a recombinant E. coli strain that carries a plasmid encoding the PvuII Gene from Proteus vulgaris. PvuII cleaves the palindromic DNA sequence CAG▼CTG.

    Concentration: 10U/µL
    Source: Proteus vulgaris
    Reagents supplied with: Buffer M and the universal Simple Buffer for double digests.
    Store at: -20 °C

    not rated 36,40 66,50 

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  • NcoI

    NcoI is a restriction enzyme purified from Nocardia corallina that cleaves the palindromic sequence CCATGG.

    Rapid digest within 15 minutes.
    Concentration: 10U/µL
    Source: Nocardia corallina
    Reagents supplied with: Buffer Simple
    The Enzyme comes with the universal Simple Buffer. The enzyme is also fully active in the universal buffers from other suppliers like the Thermo Scientific™ 10x Buffer Tango™ (Fermentas) and the CutSmart® Buffer from NEB.

    Store at: -20 °C

    not rated 49,80 219,00 

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  • KpnI

    KpnI is a restriction enzyme purified from Klebsiella pneumonia OK8 that cleaves the palindromic sequence GGTAC▼C.

    Concentration: 10U/µL
    Source: Klebsiella pneumonia OK8.
    Reagents supplied with: Buffer 10x UKpnI and 10x Simple Buffer.
    The Enzyme is also active in respective buffers from other suppliers and can combined with enzymes from other suppliers in double digests.
    Store at: -20 °C

    not rated 36,00 129,00 

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  • HindIII

    HindIII is a restriction enzyme is a restriction enzyme purified from a recombinant E. coli that carries a plasmid encoding the HindIII gene from Haemophilus influenzae. HindIII cleaves the palindromic sequence AAGCTT.

    Concentration: 10U/µL
    Source: Haemophilus influenzae Rd
    Reagents supplied with:  10x buffer M and the 10x universal Simple Buffer for double digests. The enzyme is also fully active in the respective buffers from other suppliers and may be used in double digests together with enzymes from other suppliers.
    Store at: -20 °C

    not rated 29,90 129,00 

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  • EcoRV

    EcoRV is a restriction enzyme isolated from E. coli J62plg 74 that cleaves the palindromic sequence GATATC.   

    EcoRV creates blunt ends.

    Concentration: 10U/µL

    Source: Escherichia coli, J62 plg 74

    Reagents supplied with: Buffer M and the universal Simple Buffer for double digests.

    Store at: -20 °C

    The Enzyme is supplied with 10x Buffer M (recommended) and the universal 10x Simple Buffer for double digests. The enzyme is also fully active in the respective buffers from other suppliers and can be used in double digests together with enzymes from other suppliers.

    not rated 39,60 180,00 

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  • BamHI

    BamHI is a restriction enzyme isolated from Bacillus amyloliquefaciens that cleaves the palindromic sequence G▼GATCC.

    Concentration: 10u/µL
    Source: Bacillus amyloliquefaciens H
    Reagents supplied with: The Enzyme comes with the Unique BamHI Buffer (recommended buffer) and the universal Sipmle Buffer for double digests. All our restriction buffers already contain BSA for your convenience. The enzyme is fully active in the respective buffers from other suppliers.
    Store at: -20 °C

    not rated 34,50 143,00 

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  • Analyse and Prep Agarose LEE (Molecular Biology Grade)

    If you are not satisfied with the yield or performance of your DNA gel extraction you should rather change your agarose than your extraction kit. Change now to our Simplebiotech Analyse and Prep agarose (LEE) qualified for preparative and analytic gel electrophoresis and improve the performance of your DNA extraction.

    • For analytical and preparative gel electrophoresis
    • Low Electroendosmosis  (EEO* ≤13)
    • DNase/RNAase activity not detected
    • No inhibition to restriction enzymes and ligase
    • Ensures DNA recovery without damaging its properties and structure
    • Clear and sharp bands
    • High clarity (Clarity 1.5 % (NTU) ≤ 3)
    • Extraordinary mechanical resistance for easy handling
    • Low background staining
    not rated 221,00 398,50 

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