enzyme

EcoRI is a restriction enzyme isolated from E. coli RY 13 that cleaves the palindromic sequence G▼AATTC.

Concentration: 10U/µL
Source: Escherichia coli RY13
Reagents supplied with: The Enzyme is supplied with 10x UniqueEcoRI Buffer (recommended) and the universal Simple Buffer for double digests. The enzyme is also fully active in the respective buffers from other suppliers.

Store at: -20 °C

Star activity: Conditions of low ionic strength, high enzyme concentration, glycerol concentration or pH>8.0 may result in star activity.

NheI is a restriction enzyme purified from Neisseria mucosa heidelbergensis that cleaves the palindromic sequence G▼CTAGC (ATCC 25999).

Concentration: 10U/µL
Source: Neisseria mucosa var. heidelbergensis Berger
Reagents supplied with: 10x Simple Buffer.
The Enzyme is supplied with 10x universal Simple Buffer. The Enzyme is also active in universal potassium glutamate or potassium acetate buffers from other suppliers like theCutSmart® Buffer from NEB and Tango™ Buffer from Thermo Fisher Scientific (Fermentas) and can combined with enzymes from other suppliers in double digests.
Store at: -20 °C

SstI is a restriction enzyme purified from Streptomyces stanford that cleaves the palindromic sequence GAGCT▼C.

Isoschizomer: SacI

Concentration: 10U/µL
Source: Streptomyces stanford
Reagents supplied with: 10x Buffer L and the universal 10x Simple Buffer for double digests.
Store at: -20 °C

PstI is a restriction enzyme purified from a recombinant E. coli strain that carries a plasmid encoding the PstI gene from Providencia stuartii 164. PstI cleaves the palindromic DNA sequence CTGCA^▼G.

Concentration: 10U/µL
Source: A E. coli strain that carries the cloned PstI gene from Providencia stuartii.
Reagents supplied with: Buffer UniquePstI and the universal Simple Buffer for double digests.
Store at: -20 °C