Western Blot

The chemiPRO Western Blot Imaging System is a high-performance imaging solution designed for both chemiluminescence and fluorescence-based Western blot detection. It features a highly sensitive, cooled CCD camera with high quantum efficiency, ensuring the detection of even low-abundance proteins at the picogram level. With its advanced software, chemiPRO offers precise and reproducible imaging for a wide range of applications. Additionally, when equipped with the optional transilluminator, the system is also well-suited for DNA gel imaging, making it a versatile tool for molecular biology research.
Key Features:
• Versatile Imaging Capabilities: Supports chemiluminescence blots and, with optional modules, fluorescently labelled Western blots and DNA gels.
• Highly Sensitive Cooled CCD Camera: 4-megapixel resolution with high quantum efficiency (>73% @ 425 nm) for optimal signal detection.
• Multi-Colour Fluorescence Imaging: Expandable with optional RGB and IR lighting modules for multiplex fluorescence detection.
• Automated Chemiluminescence Capture: Ensures perfect exposure without the need for film, simplifying workflow and increasing reproducibility.
• Low Background Noise: Peltier-cooled camera for excellent signal-to-noise performance and extended exposure times.
• Comprehensive Illumination Options: Includes white light LEDs for sample positioning, with optional blue, red, green, infrared, and UV lighting modules.
• Advanced Software: GenePIX for acquisition and GeneQUANT for detailed quantitative protein analysis.
• Flexible Connectivity and Compliance: Compatible with Windows systems and 21 CFR Part 11 compliant for regulated environments.
Technical Specifications:
• Image Resolution: 4 MP (effective 16 MP)
• Greyscale: 16-bit A/D conversion (65,536 greyscales)
• Maximum Image Area: 30.5 x 22.7 cm
• Optics: Motor-driven zoom lens (f/1.2)
• Cooling: Peltier system (-56°C) for enhanced image clarity
• Filter Wheel: 7-position for precise emission filtering
• Dimensions: 57 x 84 x 45 cm, Weight approx. 37 kg
• Power Supply: 115-240 V
Applications:
The chemiPRO Imaging System is optimised for Western blot imaging, supporting both chemiluminescence (e.g. ECL-based detection) and fluorescence with additional lighting modules. It is also suitable for DNA gel imaging with the optional UV transilluminator and blue LED transilluminator, as well as multiplex fluorescence, 2D gels, and IR imaging. For users requiring fluorescence imaging, it is recommended to include the necessary RGB or IR lighting modules at the time of purchase to ensure full compatibility.
Supported Applications:
• Chemiluminescence (Western blots)
• Auto-RADS DNA or RNA stained with ethidium bromide, SYPRO, SYBR, and „safe“ dyes on agarose gels
• Coomassie blue or silver-stained proteins on acrylamide gels
• Stain-free gels
• Fluorescent gels or blots stained with Qdots, DyLight, Alexa Fluor, CyDyes, and LI-COR IR dyes
• GFP samples
• Colonies or plaques on agar plates
• Bioluminescence imaging
• Plant imaging
• In vivo imaging
• 2D gels
Optional Accessories:
• RGB & IR Lighting Module – Enables fluorescence Western blot imaging with multiplex detection.
• Blue LED Transilluminator – Ideal for safe dye applications and DNA gel imaging.
• UV to Blue Light Converter – Supports DNA-safe dyes.
• Multiple Emission Filters – Customisable for different fluorescence wavelengths.
• White Light Pad & Visible Light Converter – For colourimetric applications.
• Sony Digital Thermal Printer – High-quality image documentation.
Package Contents:
• chemiPRO Imaging System
• GenePIX Acquisition Software
• GeneQUANT Analysis Software (unlimited copies)
The chemiPRO Western Blot Imaging System is a powerful and flexible solution for laboratories requiring high-sensitivity imaging of chemiluminescent and fluorescent Western blots, as well as DNA gels with the optional transilluminator. With its modular expandability, advanced software, and high-quality imaging capabilities, it is an ideal choice for researchers looking for a reliable and versatile imaging platform.