Restriktionsenzyme (synonym: Restriktionsendonukleasen) erkennen eine für das jeweilige Enzym spezifische meist palindromische Erkennungssequenz und katalysieren die Spaltung der Zucker-Phosphat-Verbindungen der DNA. In der Molekularbiologie dienen sie der Restriktionsanalyse und der DNA Klonierung.

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  • EcoRI Restriktionsenzym

    EcoRI

    EcoRI is a restriction enzyme isolated from E. coli RY 13 that cleaves the palindromic sequence G▼AATTC.

    Concentration: 10U/µL
    Source: Escherichia coli RY13
    Reagents supplied with: The Enzyme is supplied with 10x UniqueEcoRI Buffer (recommended) and the universal Simple Buffer for double digests. The enzyme is also fully active in the respective buffers from other suppliers.

    Store at: -20 °C

    Star activity: Conditions of low ionic strength, high enzyme concentration, glycerol concentration or pH>8.0 may result in star activity.

     

    not rated 36,60 143,50 

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  • NheI Restriction enzyme

    NheI

    NheI is a restriction enzyme purified from Neisseria mucosa heidelbergensis that cleaves the palindromic sequence G▼CTAGC (ATCC 25999).

    Concentration: 10U/µL
    Source: Neisseria mucosa var. heidelbergensis Berger
    Reagents supplied with: 10x Simple Buffer.
    The Enzyme is supplied with 10x universal Simple Buffer. The Enzyme is also active in universal potassium glutamate or potassium acetate buffers from other suppliers like theCutSmart® Buffer from NEB and Tango™ Buffer from Thermo Fisher Scientific (Fermentas) and can combined with enzymes from other suppliers in double digests.
    Store at: -20 °C

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  • SacI Isochizomer

    SstI (SacI)

    SstI is a restriction enzyme purified from Streptomyces stanford that cleaves the palindromic sequence GAGCT▼C.

    Isoschizomer: SacI

    Concentration: 10U/µL
    Source: Streptomyces stanford
    Reagents supplied with: 10x Buffer L and the universal 10x Simple Buffer for double digests.
    Store at: -20 °C

     

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  • SseBI StuI Isoschizomer

    SseBI (StuI)

    SseBI is a restriction enzyme purified from Streptomyces species that cleaves the palindromic sequence AGG▼CCT.

    Prototype (Isoschizomer): StuI

    Concentration: 10U/µL

    Source: Streptomyces species

    Reagents supplied with: 10x Buffer H and the universal 10x Simple Buffer for double digests.

    Store at: -20 °C

    not rated 29,40 199,90 

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  • SlaI Restriction enzyme

    SlaI (XhoI)

    SlaI is a restriction enzyme purified from Streptomyces lavendulae that cleaves the palindromic sequence CTCGAG.

    Prototype (Isoschizomer): XhoI

    Concentration: 10U/µL
    Source: Stremptomyces lavendulae
    Reagents supplied with: Buffer SH and the universal Simple Buffer for double digests. SlaI is also active in CutSmart® Buffer from NEB, 2x Tango™ Buffer from Thermo Fisher Scientific (Fermentas) or high salt buffers from other suppliers as long as the buffer is supplemented with 100µg/mL BSA.
    Store at: -20 °C

    not rated 29,20 78,70 

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  • PstI restriction endonucleases

    PstI

    PstI is a restriction enzyme purified from a recombinant E. coli strain that carries a plasmid encoding the PstI gene from Providencia stuartii 164. PstI cleaves the palindromic DNA sequence CTGCAG.

    Concentration: 10U/µL
    Source: A E. coli strain that carries the cloned PstI gene from Providencia stuartii.
    Reagents supplied with: Buffer UniquePstI and the universal Simple Buffer for double digests.
    Store at: -20 °C

     

    not rated 30,80 124,90 

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  • PvuII Restriktionsenzym

    PvuII

    PvuII is a restriction enzyme purified from a recombinant E. coli strain that carries a plasmid encoding the PvuII Gene from Proteus vulgaris. PvuII cleaves the palindromic DNA sequence CAG▼CTG.

    Concentration: 10U/µL
    Source: Proteus vulgaris
    Reagents supplied with: Buffer M and the universal Simple Buffer for double digests.
    Store at: -20 °C

    not rated 36,40 66,50 

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  • EcoRV

    EcoRV is a restriction enzyme isolated from E. coli J62plg 74 that cleaves the palindromic sequence GATATC.   

    EcoRV creates blunt ends.

    Concentration: 10U/µL

    Source: Escherichia coli, J62 plg 74

    Reagents supplied with: Buffer M and the universal Simple Buffer for double digests.

    Store at: -20 °C

    The Enzyme is supplied with 10x Buffer M (recommended) and the universal 10x Simple Buffer for double digests. The enzyme is also fully active in the respective buffers from other suppliers and can be used in double digests together with enzymes from other suppliers.

    not rated 39,60 180,00 

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  • BamHI restriction endonuclease

    BamHI

    BamHI is a restriction enzyme isolated from Bacillus amyloliquefaciens that cleaves the palindromic sequence G▼GATCC.

    Concentration: 10u/µL
    Source: Bacillus amyloliquefaciens H
    Reagents supplied with: The Enzyme comes with the Unique BamHI Buffer (recommended buffer) and the universal Sipmle Buffer for double digests. All our restriction buffers already contain BSA for your convenience. The enzyme is fully active in the respective buffers from other suppliers.
    Store at: -20 °C

    not rated 34,50 143,00 

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