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  • SseBI StuI Isoschizomer

    SseBI (StuI)

    SseBI is a restriction enzyme purified from Streptomyces species that cleaves the palindromic sequence AGG▼CCT.

    Prototype (Isoschizomer): StuI

    Concentration: 10U/µL

    Source: Streptomyces species

    Reagents supplied with: 10x Buffer H and the universal 10x Simple Buffer for double digests.

    Store at: -20 °C

    not rated 29,40 199,90 

    exkl. MwSt.

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    Lieferzeit: Lieferzeit 1-2 Werktage

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  • T4 DNA Ligase for DNA klonierung Polydeoxyribonucleotide synthase

    T4 DNA Ligase

    T4 DNA Ligase (2.5 Weiss*units/μL and 6.0 Weiss/units/uL)

    • T4 DNA Ligase catalyses the formation of a phosphodiester bond between neighbouring 5′ -phosphate and 3′ -hydroxyl termini in duplex DNA or RNA
    • Covalently joins DNA fragments with complementary cohesive ends or blunt ends
    • Cloning of PCR and restriction fragments
    • Self circularisation of linear DNA
    • Ligation of adaptors or linkers to DNA
    • Repair of single stranded breaks (nicks)

     

    (*Note: one Weiss unit is equivalent to about 67 cohesive end ligation units)

    not rated 36,30 64,20 

    exkl. MwSt.

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    Lieferzeit: Lieferzeit 1-2 Werktage

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  • Scai restriction endonuclease

    ScaI

    ScaI is a restriction enzyme purified from Streptomyces caespitosus that cleaves the palindromic sequence AGT▼ACT creating blunt ends.

    Concentration: 10U/µL
    Source: Streptomyces caespitosus
    Reagents supplied with: Buffer 10x UScaI
    The Enzyme is also active in respective buffers from other suppliers and can combined with enzymes from other suppliers in double digests.
    Store at: -20 °C

    not rated 33,50 132,80 

    exkl. MwSt.

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    Lieferzeit: Lieferzeit 1-2 Werktage

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  • PvuII Restriktionsenzym

    PvuII

    PvuII is a restriction enzyme purified from a recombinant E. coli strain that carries a plasmid encoding the PvuII Gene from Proteus vulgaris. PvuII cleaves the palindromic DNA sequence CAG▼CTG.

    Concentration: 10U/µL
    Source: Proteus vulgaris
    Reagents supplied with: Buffer M and the universal Simple Buffer for double digests.
    Store at: -20 °C

    not rated 36,40 66,50 

    exkl. MwSt.

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    Lieferzeit: Lieferzeit 1-2 Werktage

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  • EcoRV

    EcoRV is a restriction enzyme isolated from E. coli J62plg 74 that cleaves the palindromic sequence GATATC.   

    EcoRV creates blunt ends.

    Concentration: 10U/µL

    Source: Escherichia coli, J62 plg 74

    Reagents supplied with: Buffer M and the universal Simple Buffer for double digests.

    Store at: -20 °C

    The Enzyme is supplied with 10x Buffer M (recommended) and the universal 10x Simple Buffer for double digests. The enzyme is also fully active in the respective buffers from other suppliers and can be used in double digests together with enzymes from other suppliers.

    not rated 39,60 180,00 

    exkl. MwSt.

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    Lieferzeit: Lieferzeit 1-2 Werktage

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  • Quick Ligation Kit Simplebiotech Schnellligations Kit

    Rapid DNA Ligation Kit

    Rapid DNA Ligation Kit for fast cloning at affordable price

    • Quick: DNA ligation of blunt or sticky DNA ends in just 15min or less
    • Convienient ligation at room temperature
    • Improved ligation of blunt end DNA
    • Direct transformation of chemical competent e. coli cells 

    (For transformation of electrocometent e. coli cells the ligation product should be purifed before transformation)

    not rated 49,90 78,40 

    exkl. MwSt.

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    Lieferzeit: Lieferzeit 1-2 Werktage

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