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not rated 29,40 € – 199,90 €SseBI (StuI)
SseBI is a restriction enzyme purified from Streptomyces species that cleaves the palindromic sequence AGG▼CCT.
Prototype (Isoschizomer): StuI
Concentration: 10U/µL
Source: Streptomyces species
Reagents supplied with: 10x Buffer H and the universal 10x Simple Buffer for double digests.
Store at: -20 °C
not rated 36,30 € – 64,20 €T4 DNA Ligase
T4 DNA Ligase (2.5 Weiss*units/μL and 6.0 Weiss/units/uL)
- T4 DNA Ligase catalyses the formation of a phosphodiester bond between neighbouring 5′ -phosphate and 3′ -hydroxyl termini in duplex DNA or RNA
- Covalently joins DNA fragments with complementary cohesive ends or blunt ends
- Cloning of PCR and restriction fragments
- Self circularisation of linear DNA
- Ligation of adaptors or linkers to DNA
- Repair of single stranded breaks (nicks)
(*Note: one Weiss unit is equivalent to about 67 cohesive end ligation units)
not rated 33,50 € – 132,80 €ScaI
ScaI is a restriction enzyme purified from Streptomyces caespitosus that cleaves the palindromic sequence AGT▼ACT creating blunt ends.
Concentration: 10U/µL
Source: Streptomyces caespitosus
Reagents supplied with: Buffer 10x UScaI
The Enzyme is also active in respective buffers from other suppliers and can combined with enzymes from other suppliers in double digests.
Store at: -20 °C
not rated 36,40 € – 66,50 €PvuII
PvuII is a restriction enzyme purified from a recombinant E. coli strain that carries a plasmid encoding the PvuII Gene from Proteus vulgaris. PvuII cleaves the palindromic DNA sequence CAG▼CTG.
Concentration: 10U/µL
Source: Proteus vulgaris
Reagents supplied with: Buffer M and the universal Simple Buffer for double digests.
Store at: -20 °C
not rated 39,60 € – 180,00 €EcoRV
EcoRV is a restriction enzyme isolated from E. coli J62plg 74 that cleaves the palindromic sequence GAT▼ATC.
EcoRV creates blunt ends.
Concentration: 10U/µL
Source: Escherichia coli, J62 plg 74
Reagents supplied with: Buffer M and the universal Simple Buffer for double digests.
Store at: -20 °C
The Enzyme is supplied with 10x Buffer M (recommended) and the universal 10x Simple Buffer for double digests. The enzyme is also fully active in the respective buffers from other suppliers and can be used in double digests together with enzymes from other suppliers.
not rated 49,90 € – 78,40 €Rapid DNA Ligation Kit
Rapid DNA Ligation Kit for fast cloning at affordable price
- Quick: DNA ligation of blunt or sticky DNA ends in just 15min or less
- Convienient ligation at room temperature
- Improved ligation of blunt end DNA
- Direct transformation of chemical competent e. coli cells
(For transformation of electrocometent e. coli cells the ligation product should be purifed before transformation)
