DNA cloning or molecular cloning means the assembly of recombinant DNA molecules and their propagation in the host organism. One example is the insertion of a human DNA fragment (gene) into a plasmid and its duplication in E. coli.

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  • T4 DNA Ligase for DNA klonierung Polydeoxyribonucleotide synthase

    T4 DNA Ligase

    T4 DNA Ligase (2.5 Weiss*units/μL and 6.0 Weiss/units/uL)

    • T4 DNA Ligase catalyses the formation of a phosphodiester bond between neighbouring 5′ -phosphate and 3′ -hydroxyl termini in duplex DNA or RNA
    • Covalently joins DNA fragments with complementary cohesive ends or blunt ends
    • Cloning of PCR and restriction fragments
    • Self circularisation of linear DNA
    • Ligation of adaptors or linkers to DNA
    • Repair of single stranded breaks (nicks)

     

    (*Note: one Weiss unit is equivalent to about 67 cohesive end ligation units)

    not rated 36,30 64,20 

    exkl. MwSt.

    zzgl. Versandkosten

    Lieferzeit: Lieferzeit 1-2 Werktage

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  • Quick Ligation Kit Simplebiotech Schnellligations Kit

    Rapid DNA Ligation Kit

    Rapid DNA Ligation Kit for fast cloning at affordable price

    • Quick: DNA ligation of blunt or sticky DNA ends in just 15min or less
    • Convienient ligation at room temperature
    • Improved ligation of blunt end DNA
    • Direct transformation of chemical competent e. coli cells 

    (For transformation of electrocometent e. coli cells the ligation product should be purifed before transformation)

    not rated 49,90 78,40 

    exkl. MwSt.

    zzgl. Versandkosten

    Lieferzeit: Lieferzeit 1-2 Werktage

    Details