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not rated 36,30 € – 64,20 €T4 DNA Ligase
T4 DNA Ligase (2.5 Weiss*units/μL and 6.0 Weiss/units/uL)
- T4 DNA Ligase catalyses the formation of a phosphodiester bond between neighbouring 5′ -phosphate and 3′ -hydroxyl termini in duplex DNA or RNA
- Covalently joins DNA fragments with complementary cohesive ends or blunt ends
- Cloning of PCR and restriction fragments
- Self circularisation of linear DNA
- Ligation of adaptors or linkers to DNA
- Repair of single stranded breaks (nicks)
(*Note: one Weiss unit is equivalent to about 67 cohesive end ligation units)
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not rated 49,90 € – 78,40 €Rapid DNA Ligation Kit
Rapid DNA Ligation Kit for fast cloning at affordable price
- Quick: DNA ligation of blunt or sticky DNA ends in just 15min or less
- Convienient ligation at room temperature
- Improved ligation of blunt end DNA
- Direct transformation of chemical competent e. coli cells
(For transformation of electrocometent e. coli cells the ligation product should be purifed before transformation)
