Ergebnisse 73 – 80 von 80 werden angezeigt

  • Quick Ligation Kit Simplebiotech Schnellligations Kit

    Rapid DNA Ligation Kit

    Rapid DNA Ligation Kit for fast cloning at affordable price

    • Quick: DNA ligation of blunt or sticky DNA ends in just 15min or less
    • Convienient ligation at room temperature
    • Improved ligation of blunt end DNA
    • Direct transformation of chemical competent e. coli cells 

    (For transformation of electrocometent e. coli cells the ligation product should be purifed before transformation)

    not rated 49,90 78,40 

    exkl. MwSt.

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    Lieferzeit: Lieferzeit 1-2 Werktage

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  • Adenosin 5' Triphosphate

    ATP (Adenosine 5´-Triphosphate), 100mM

    ATP (Adenosine 5′-Triphosphate, 100mM

                >98 % Purity

                Adjusted to pH 7.5 with Tris base (can be stored at -20 °C for 12 month)

    not rated 49,20 

    exkl. MwSt.

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    Lieferzeit: Lieferzeit 1-2 Werktage

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  • Klenow Enzyme (DNA Polymerase I Large Fragment)

    Concentration: 5U/µL

    • Highly pure recombinat enzyme (>98 % as indicated by SDS-PAGE)
    • Lacks the 5´->3´ exonuclease activity
    • Retains polymerase and 3’→5’exonuclease activity (proofreading, blunting)
    • Fill-in of 5’ overhangs to form blunt ends (blunting)
    • Second strand cDNA synthesis
    • Probe labelling, using random primers

    Source: E. coli strain carrying a plasmid with a clone copy of DNA Polymerase I large fragment
    Reagents supplied with: 10x Klenow reaction buffer
    Store at: -20 °C

    not rated 42,10 169,50 

    exkl. MwSt.

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    Lieferzeit: Lieferzeit 1-2 Werktage

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  • Scai restriction endonuclease

    ScaI

    ScaI is a restriction enzyme purified from Streptomyces caespitosus that cleaves the palindromic sequence AGT▼ACT creating blunt ends.

    Concentration: 10U/µL
    Source: Streptomyces caespitosus
    Reagents supplied with: Buffer 10x UScaI
    The Enzyme is also active in respective buffers from other suppliers and can combined with enzymes from other suppliers in double digests.
    Store at: -20 °C

    not rated 33,50 132,80 

    exkl. MwSt.

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  • PvuII Restriktionsenzym

    PvuII

    PvuII is a restriction enzyme purified from a recombinant E. coli strain that carries a plasmid encoding the PvuII Gene from Proteus vulgaris. PvuII cleaves the palindromic DNA sequence CAG▼CTG.

    Concentration: 10U/µL
    Source: Proteus vulgaris
    Reagents supplied with: Buffer M and the universal Simple Buffer for double digests.
    Store at: -20 °C

    not rated 36,40 66,50 

    exkl. MwSt.

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  • KpnI restriction enzyme

    KpnI

    KpnI is a restriction enzyme purified from Klebsiella pneumonia OK8 that cleaves the palindromic sequence GGTAC▼C.

    Concentration: 10U/µL
    Source: Klebsiella pneumonia OK8.
    Reagents supplied with: Buffer 10x UKpnI and 10x Simple Buffer.
    The Enzyme is also active in respective buffers from other suppliers and can combined with enzymes from other suppliers in double digests.
    Store at: -20 °C

    not rated 36,00 129,00 

    exkl. MwSt.

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  • EcoRV

    EcoRV is a restriction enzyme isolated from E. coli J62plg 74 that cleaves the palindromic sequence GATATC.   

    EcoRV creates blunt ends.

    Concentration: 10U/µL

    Source: Escherichia coli, J62 plg 74

    Reagents supplied with: Buffer M and the universal Simple Buffer for double digests.

    Store at: -20 °C

    The Enzyme is supplied with 10x Buffer M (recommended) and the universal 10x Simple Buffer for double digests. The enzyme is also fully active in the respective buffers from other suppliers and can be used in double digests together with enzymes from other suppliers.

    not rated 39,60 180,00 

    exkl. MwSt.

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    Lieferzeit: Lieferzeit 1-2 Werktage

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  • Agarose Analyse and Prep (Molecular Biology Grade) LEE

    If you are not satisfied with the yield or performance of your DNA gel extraction you should rather change your agarose than your extraction kit. Change now to our Simplebiotech Analyse and Prep agarose (LEE) qualified for preparative and analytic gel electrophoresis and improve the performance of your DNA extraction.

    • For analytical and preparative gel electrophoresis
    • Low Electroendosmosis  (EEO* ≤13)
    • DNase/RNAase activity not detected
    • No inhibition to restriction enzymes and ligase
    • Ensures DNA recovery without damaging its properties and structure
    • Clear and sharp bands
    • High clarity (Clarity 1.5 % (NTU) ≤ 3)
    • Extraordinary mechanical resistance for easy handling
    • Low background staining
    not rated 221,00 398,50 

    exkl. MwSt.

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    Lieferzeit: Lieferzeit 1-2 Werktage

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