Agarosen

Analyse and Prep Agarose LEE (Molecular Biology Grade)

221,00 398,50 

If you are not satisfied with the yield or performance of your DNA gel extraction you should rather change your agarose than your extraction kit. Change now to our Simplebiotech Analyse and Prep agarose (LEE) qualified for preparative and analytic gel electrophoresis and improve the performance of your DNA extraction.

  • For analytical and preparative gel electrophoresis
  • Low Electroendosmosis  (EEO* ≤13)
  • DNase/RNAase activity not detected
  • No inhibition to restriction enzymes and ligase
  • Ensures DNA recovery without damaging its properties and structure
  • Clear and sharp bands
  • High clarity (Clarity 1.5 % (NTU) ≤ 3)
  • Extraordinary mechanical resistance for easy handling
  • Low background staining

Lieferzeit: Lieferzeit 1-2 Werktage

Artikelnummer: #RHA1 Kategorien: , , Schlüsselworte: , , ,

Beschreibung

Chemical and physical properties:

Appearance

Fine, homogeneous powder

Colour

white

Moisture

4.98 %

Ash

0.39

Electroendoosmosis -Mr

pH 8.4, Wieme method

0.12

Sulfate

0.098 %

Gel strength 1%

1,230g/cm2

Gel strength 1,5%

2,805g/cm2

Clarity 1.5% (NTU)

2.98

pH in solution

7.20

Colourimetry (Absorbance)

nmu 450

0.037

Gelling temprature

36.9 °C

Melting temperature

89.0 °C

DNase and RNase

None detected

 

Functionally tested for:

-Background fluorescence assay in Ethidium Bromide

-Comparative assay of different size DNA fragments

-Southern Blotting

-DNA binding

-Restriction and Ligation Assay (assayed enzymes: BamHI, EcoRI, HindIII, HindII and T4-DNA-Ligase)

-DNAse/RNAse activity: none detected

References:

Ogden, Richard C., and Deborah A. Adams. “[8] Electrophoresis in agarose and acrylamide gels.” Methods in enzymology. Vol. 152. Academic Press, 1987. 61-87.

 

 

agarose concentration for different DNA fragment sizes

Fragment lenth [kb]Agarose concentration [percent]
0.1 - 22
0.2 - 31.5
0.4 - 61.2
0.5 - 70.9
0.8 - 100.7
1 - 200.6
5 - 600.3

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