Beschreibung
Chemical and physical properties:
|
Appearance |
Fine, homogeneous powder |
|
Colour |
white |
|
Moisture |
4.98 % |
|
Ash |
0.39 |
|
Electroendoosmosis -Mr pH 8.4, Wieme method |
0.12 |
|
Sulfate |
0.098 % |
|
Gel strength 1% |
1,230g/cm2 |
|
Gel strength 1,5% |
2,805g/cm2 |
|
Clarity 1.5% (NTU) |
2.98 |
|
pH in solution |
7.20 |
|
Colourimetry (Absorbance) nmu 450 |
0.037 |
|
Gelling temprature |
36.9 °C |
|
Melting temperature |
89.0 °C |
|
DNase and RNase |
None detected |
Functionally tested for:
-Background fluorescence assay in Ethidium Bromide
-Comparative assay of different size DNA fragments
-Southern Blotting
-DNA binding
-Restriction and Ligation Assay (assayed enzymes: BamHI, EcoRI, HindIII, HindII and T4-DNA-Ligase)
-DNAse/RNAse activity: none detected
References:
Ogden, Richard C., and Deborah A. Adams. „[8] Electrophoresis in agarose and acrylamide gels.“ Methods in enzymology. Vol. 152. Academic Press, 1987. 61-87.
agarose concentration for different DNA fragment sizes
| Fragment lenth [kb] | Agarose concentration [percent] |
|---|---|
| 0.1 - 2 | 2 |
| 0.2 - 3 | 1.5 |
| 0.4 - 6 | 1.2 |
| 0.5 - 7 | 0.9 |
| 0.8 - 10 | 0.7 |
| 1 - 20 | 0.6 |
| 5 - 60 | 0.3 |
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