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6x Orange DNA Loading Buffer

9,20 18,50 

Store at room temperature for up to 12 months. For longer periods, store at -20°C.

 

6x Orange DNA Loading Buffer

Eco-friendly DNA loading dye

• Free from persistent organohalogen compounds like and bromophenol blue and and the common desity reagent saccharose-epichlorhydrin-copolymer.
• The Orange G tracking dye, comigrates with approximately 50bp dsDNA on 1% TAE or TBE Gel
• No DNA masking on UV or blue light
• SDS efficiently stops restriction digests and nucleases
• Reduced EDTA content for clear and sharp bands
• Optimised glycerol content for easy loading

Lieferzeit: Lieferzeit 1-2 Werktage

Artikelnummer: #SR4 Kategorien: , ,

Beschreibung

Composition
30% (V/V) Glycerol, 40 mM Tris-acetate pH 8.3 at 25 °C, 1 mM EDTA, 0.6 % (m/V) SDS, 0.25% (m/V) Orange G

Internal tests revealed that high EDTA concentrations, necessary for stopping restriction digests are correlated with poorly focused bands.

The Simplebiotech 6x Orange DNA loading buffer contains only 1mM EDTA for improved buffer stability during storage. Our 6x Orange DNA Loading buffer contains 0.6 % SDS instead, which efficiently stops most enzymatic reactions.

SDS eliminates DNA interaction with restriction enzymes and DNA binding proteins providing clear and sharp bands. It also suppresses the appearance of additional bands caused by cohesive end annealing.

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