Restriction Enzymes

BglII

34,70 143,20 

Bgl II is a restriction enzyme that cleaves the palindromic sequence A▼GATCT. The Enzyme is purified from a Bacillus globigii strain lacking Bgl I.

Concentration: 10U/µL

Reagents supplied with: The Enzyme is supplied with 10x Buffer H and the universal 10x Simple Buffer for double digests. The Enzyme is also active in the respective buffers from other suppliers can be combined with enzymes from other suppliers in double digests.
Store at: -20 °C

Lieferzeit: Lieferzeit 1-2 Werktage

Artikelnummer: #RM2 Kategorien: ,

Beschreibung

Unit definition: One unit is defined as amount of enzyme required to produce a complete digest of 1 µg Lambda DNA in 60 min in a total reaction volume of 50µL under the assay conditions.
Unit substrate: Lambda DNA
Unit calculation assay conditions: 100 mM NaCl, 50 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl2, 1mM DTT, 100 μg/ml BSA. Incubation temperature 37 °C
Absence of contaminants: 50 units of Bgl II do not produce any unspecific cleavage products after 16 hrs incubation with 1µg of Lambda DNA at 37 °C. After 50-fold overdigestion with Bgl II greater than 95% of the DNA fragments can be ligated and recleaved with this enzyme.
Storage buffer: Bgl II is supplied in 50 mM KCl, 10 mM Tris HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml bovine serum albumin and 50% glycerol.
Inactivation: Bgl II cannot be heat inactivated in 20min at 80 °C. The enzyme can be removed by spin column purification. It is also possible to stop the digest by addition of EDTA to a final concentration of >10mM or SDS to a concentration of 0.1 %. Alternatively use our environmentally friendly 6x orange gel loading buffer. For further enzymatic reactions remove SDS or EDTA by spin column-based PCR purification kit.
Methylation Sensitivity:
dam methylation: Not sensitive
dcm methylation: Not sensitive
CpG Methylation: Not sensitive

Activity in Simplebiotech buffers:

LMHSHSimple2x Simple
107510075-10075-100100

 

10x Buffer H: 1000mM NaCl, 500mM Tris-HCl (pH 7.9 at 25 °C), 100mM MgCl2, 1mg/mL BSA
10x Simple buffer: 500 mM Potassium Acetate, 200 mM Tris-acetate (pH 7.9 at 25°C), 100 mM Magnesium Acetate, 1000 μg/ml BSA

Rapid digest within 15 minutes.                                                              

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