The Simplebiotech Taq Polymerase is purified from an E. coli strain carrying a plasmid with Taq DNA polymerase gene from Thermus aquaticus YT-1.
Description: Taq DNA Polymerase is a thermostable enzyme that catalyses 5’→3′ synthesis of DNA. The enzyme has no 3’→5’ proofreading exonuclease activity but possesses low 5’→3′ exonuclease activity.
Unit definition: One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nmoles of dNTPs into acid insoluble material in 30 minutes at 72 °C.
Assay conditions: The polymerase activity was assayed in 10 mM Tris-HCl (pH 8.5 at 25 °C), 50mM KCl, 1.5 mM MgCl2, 200 µM each of dATP, dGTP, dCTP, dTTP (a mix of unlabelled and [3H] dTTP) and 12.5 µg activated calf thymus DNA, in a final volume of 50 µL.
Storage buffer: 100 mM NaCl, 50 mM Tris-HCl (pH 8.0 at 25 °C), 1mM DTT, 0.1 mM EDTA, 1% Triton X-100 and 50% Glycerol.
Taq DNA polymerase buffer: 50 mM KCl, 10 mM Tris-HCl (pH 8.5 at 25 °C) 1.5 mM MgCl2, 0.1 % Triton X-100.
Our Taq DNA Polymerase has been extensively functionally tested and carefully tested for absence of endo- and exodeoxyribonucleases.
The PCR process is disclosed and claimed in European patents EP0201184B1 and EP0200362B1 or United States patent US4965188A, which have expired along with all family members/equivalents in all territories. However, some specific product applications may fall within the scope of protection of granted patents, which may be in force in the United States, European Union and certain countries. The purchase of this product does not include a license to perform any application subject to patent protection, it is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties.
For Research Use Only. Not for use in diagnostic procedures.