Bovine Serum Albumin (BSA), non-acetylated
molecular biology grade 20mg/mL
Description: Highly pure non-acetylated not chemically modified BSA for use as enzyme stabiliser. Stabilises many enzymes including restriction enzymes, T4-Ligase and DNA-Polymerases during storage and incubation and reduces adhesion to reaction tubes. Unlike acetylated BSA that inhibits PCR 1 amplification, non-acetylated BSA improves PCR amplification of low purity templates 2 and is reported to enhance PCR of GC rich templates amplification in conjunction with DMSO or formamide 3.
Storage Buffer: 10 mM Tris-HCl (pH 7.5 at 25 °C), 100mM KCl, 0.5 mM EDTA and 50 % glycerol.
Usage: The standard concentration for use as enzyme stabiliser is 0.2mg/mL. The standard concentration for use as PCR enhancer is 0.4mg/mL, but concentrations between 1mg/mL and up to 10mg/mL have been successfully used as co-enhancer in PCR together with DMSO or formamide.
Note: This product is not recommended as mass standard for protein determination or size standard for protein electrophoresis.
Absence of DNase: No degradation was observed by agarose gel electrophoresis after 24h incubation of linear DNA and supercoiled plasmid DNA with 2mg BSA at 37 °C.
Functional Assay: Functionally tested in PCR with Taq Polymerase.
1) Chang, B. S., & Mahoney, R. R. (1995). Enzyme thermostabilization by bovine serum albumin and other proteins: evidence for hydrophobic interactions. Biotechnology and applied biochemistry, 22, 203-214.
2) McKeown, B. J. (1994). An acetylated (nuclease-free) bovine serum albumin in a PCR buffer inhibits amplification. BioTechniques, 17(2), 246.
3) Garland, S., Baker, A., Phillott, A. D., & Skerratt, L. F. (2010). BSA reduces inhibition in a TaqMan® assay for the detection of Batrachochytrium dendrobatidis. Diseases of Aquatic Organisms, 92, 113-116.
4) Farell, E. M., & Alexandre, G. (2012). Bovine serum albumin further enhances the effects of organic solvents on increased yield of polymerase chain reaction of GC-rich templates. BMC research notes, 5(1), 257.
5) Yoshino, Y., Ishida, M., & Horii, A. (2007). A new 10-min ligation method using a modified buffer system with a very low amount of T4 DNA ligase: the “Coffee Break Ligation” technique. Biotechnology letters, 29(10), 1557-1560.